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Proteomics Core

Co-Directors: Rick Edmondson, PhD, Associate Professor of Medicine and Sam Mackintosh, PhD, Research Assistant Professor, Department of Biochemistry and Molecular Biology

Core Staff: Renny Lan, PhD, Instructor, Department of Biochemistry and Molecular Biology

Technician: Lisa Orr, BS

Postdoctoral Fellow: Aaron Storey, PhD

Purpose

The overall theme of the CTPR is to study how pediatric diseases develop from systems biology and mechanistic standpoints with the ultimate goal of identifying points in the intersection of disease and development which, in turn, will produce targets for intervention and the development of new treatments. The Proteomics Core provides a crucial piece of the systems biology puzzle - the large-scale identification and quantification of proteins and protein posttranslational modifications.

Services Offered

  • Protein identification
  • Mapping of posttranslational modifications
  • Label-free protein quantification
  • Offline high pH reverse phase peptide fractionation
  • SILAC (stable isotope labeling by amino acids in cell culture)
  • Custom service

Equipment

  • Fusion Lumos Tribrid Mass Spectrometer
  • Oribitrap Fusion Tribrid Mass Spectrometer
  • LTQ Orbitrap Velos Mass Spectrometer
  • nanoAcquity Ultra Performance Liquid Chromatography (UPLC)
  • UltiMate 3000 Binary Analytical LC System

NIH Equipment and Facilities/Resources Information

Contact

https://uams.corefacilities.org//service_center/show_external/4155